| Does PNA Bio provide a warranty for their products? |
| Yes, PNA Bio stands fully behind the quality of their products. If any quality issue arises, PNA Bio will provide a free replacement and, if necessary, a refund if the replacement also does not work. |
| Why should you order custom PNA oligomers from PNA Bio? |
| As a specialized PNA company, PNA Bio is dedicated to delivering high-quality products, offering >95% purity after HPLC purification and including mass data for all products. |
| Has the Cas9 protein from PNA Bio been used for embryo injection? |
| Yes, our Cas9 protein has been successfully used for embryo injection in several species, including mice, zebrafish, rats, Xenopus, butterflies, mosquitoes, C. elegans, and fruit flies. |
| Can PNA Bio's Cas9 protein be used for transfection to generate KO and KI cell lines? |
| Yes, PNA Bio's Cas9 protein includes two NLS for nuclear transport and is endotoxin-free, making it suitable for use in any cell type or embryo injection. |
| If I order Cas9 protein today, when will I receive it? |
| All Cas9 protein products are in stock and ship the same day if the order is placed by 5 pm ET, for the next-day delivery. |
| Are the Cas9 proteins in lyophilized or liquid form? |
| All Cas9 protein products are lyophilized except for CP04 products. |
| How is the Cas9 protein shipped and how long is it stable? |
| Our lyophilized Cas9 protein is shipped at room temperature and should be stable for at least 2 weeks under these conditions. |
| What is the quality assurance for PNA oligomers synthesized by PNA Bio? |
| All PNA products are analyzed by HPLC for purity and by MALDI-TOF for mass confirmation, ensuring a thorough synthesis process. Most PNA probes are provided with >95% purity. |
| Does PNA Bio offer custom PNA conjugation to peptides or chemicals? |
| Yes, PNA Bio can conjugate peptides or other chemicals to PNA via amide linkage or SMCC ligation. |
| Can you help design PNA against my targets? |
| Yes, the PNA Bio team can review your sequence or design PNA probes for your taget at no additional charge. |
| How can labeled PNA be used to quantify siRNA for pharmacokinetics studies? |
| PNA conjugated with fluorescent dyes or biotin can capture short DNA or RNA targets like siRNA due to its high affinity. The resulting complex can then be analyzed by HPLC for quantification. |
| Can PNA Bio provide a labeled PNA with a specific fluorophore? Can it be labeled at the 5' or 3' end? |
| Most NHS ester-form fluorophores can be conjugated to PNA at either end. For 3' end labeling, one lysine is added. |
| Why are PNA telomere and PNA centromere probes superior for telomere FISH and centromere FISH? |
| With high affinity and specificity, PNA telomere and centromere probes are highly effective for FISH applications, with faster hybridization times (1–2 hours) than conventional DNA or RNA probes. |
| What is the maximum length of PNA that PNA Bio can synthesize? |
| PNA Bio can synthesize PNA up to approximately 50mer, depending on the sequence. |
| Why is PNA oligo superior to DNA for hybridization? |
| PNA’s backbone is composed of methylene carbonyl bonds, like peptides, and lacks charged phosphate groups, resulting in stronger binding to DNA due to reduced electrostatic repulsion. PNA is also resistant to DNases and proteases and remains stable across a wide pH and salt range. |
| How is surrogate reporter system help to select recombination event? |
| Our
reporter plasmid contains GFP in out of frame that becomes in frame by
recombination event carried by active recombination event. By selecting
the cells with GFP expression, the chance to find a cell with
recombination improves 10~50 fold. |