PNA FISH Probes

PNA probes hybridize to target DNA with high sensitivity and specificity mainly due to electrical neutrality of its chemical structure. This trait enables PNA probes to be more efficient and useful in applying to FISH (fluorescent in situ hybridization) even at low concentrations. Hybridization occurs fast (within a couple of hours) and background noise is reduced significantly.
PNA Bio offers telomere, centromere, and CAG repeat probes as catalog itmes. If you need custom detection, we can provide custom PNA probe synthesis service.

TelC is C-rich a telomere probe for a leading strand (repeats of TAACCC) and TelG is a G-rich telomere probe for lagging strand (repeats of TTAGGG). Both work with human, mouse and rat chromosomes.

CENPB probe is a binding site for centromere protein B (CENP-B), one of the major centromere specific proteins. The probe sequence is ATTCGTTGGAAACGGGA, and it stains minor satellite of all human and mouse centromere except Y chromosome.

Cent probe is specific for human alpha satellite centromere and does not react to mouse.
The sequence of the probe is AAACTAGACAGAAGCAT.
For FISH protocol using PNA FISH probes, please download following file.

F1001 (TelC-FAM)
F1002 (TelC-Cy3)
F1002 & F3001 (CENPB-FAM)
F1006 (TelG-Cy3)
F3006 (Cent-FAM)
F5001 (CAG5-Cy3)

Catalog Number Item Label Information
F1001 TelC-FAM FAM
TelC telomere probe
(CCCTAACCCTAACCCTAA)
C-rich/leading strand
Reacts to human, mouse, rat, etc

F1002 TelC-Cy3 Cy3
F1003 TelC-Cy5 Cy5
F1004 TelC-Alexa488 Alexa Fluor 488
F1009 TelC-FITC FITC
F1013 TelC-Alexa647 Alexa Fluor 647
F2001 TelC-Biotin Biotin
F1005 TelG-FAM FAM
TelG telomere probe
(TTAGGGTTAGGGTTAGGG)
G-rich/lagging strand
Reacts to human, mouse, rat, etc

F1006 TelG-Cy3 Cy3
F1007 TelG-Cy5 Cy5
F1008 TelG-Alexa488 Alexa Fluor 488
F1010 TelG-FITC FITC
F1014 TelG-Alexa 647 Alexa Fluor 647
F1020 aTelC-Alexa488 Alexa Fluor 488 TelC probe for arthropods (CCTAA repeats)
F3001 CENPB-FAM FAM
Pan-centromere probe
(ATTCGTTGGAAACGGGA)
Reacts to human & mouse
F3002 CENPB-Cy3 Cy3
F3004 CENPB-Alexa488 Alexa Fluor 488
F3005 CENPB-Cy5 Cy5
F3008 CENPB-Biotin Biotin
F3003 CENT-Cy3 Cy3
Human only pan-centromere probe
(AAACTAGACAGAAGCATT)
F3006 CENT-FAM FAM
F5001 (CAG)5-Cy3 Cy3 Stains CAG repeats (DM-1)

* FAM and Alexa488 is more stable alternative for FITC.

* Cy3 is more stable alternative for Texas Red, TRITC, TMR and other red dyes.
  1. Integrity of the human centromere DNA repeats is protected by CENP-A, CENP-C, & CENP-T . Giunta S & Funabiki H. (2017) Proc Natl Acad Sci USA. In press.
  2. Comprehensive characterization of neutrophil genome topology. Zhu Y et al. (2017) Genes & Development. In press.
  3. Long telomeres protect against age-dependent cardiac disease caused by NOTCH1 haploinsufficiency. Theodoris CV et al. (2017) J Clinical Investigation In press.
  4. Quantification of telomere features in tumor tissue sections by an automated 3D imaging-based workflow. Gunkel M et al (2017) Methods 114:60-73.
  5. Telomere Replication Stress Induced by POT1 Inactivation Accelerates Tumorigenesis. Pinzaru AM et al. (2016) Cell Rep 15(10):2170-84.
  6. Regulation of the Human Telomerase Gene TERT by Telomere Position Effect—Over Long Distances (TPE-OLD): Implications for Aging and Cancer. Kim W et al (2016) pLoS Biology 2000016.
  7. Dysfunctional telomeres induce p53‐dependent and independent apoptosis to compromise cellular proliferation and inhibit tumor formation. Wang Y et al (2016) Aging Cell 15(4): 646–660.
  8. ATRX loss promotes tumor growth and impairs nonhomologous end joining DNA repair in glioma. Koschmann C et al (2016) Sci Transl Med. 8(328):328.
  9. Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis. Elkouby YM et al. (2016) PLoS Biol. 14(1):e1002335.
  10. Cell Death During Crisis Is Mediated by Mitotic Telomere Deprotection. Hayashi MT et al (2015)Nature 522(7557): 492–496.
  11. SMARCAL1 maintains telomere integrity during DNA replication. Poole LA et al. (2015) Proc Natl Acad Sci U S A. 112(48):14864-9.
  12. Visualization and quantitative analysis of extrachromosomal telomere-repeat DNA in individual human cells by Halo-FISH. Komosa M et al. (2015)Nucleic Acids Res. 43(4): 2152–2163.
  13. Flap Endonuclease 1 Limits Telomere Fragility on the Leading Strand.Teasley DC et al (2015) J Biol Chem. 290(24):15133-45.
  14. Replication Stress and Telomere Dysfunction Are Present in Cultured Human Embryonic Stem Cells. Janson C et al (2015) Cytogenet Genome Res. 146(4):251-60.
  15. Genomic mosaicism with increased amyloid precursor protein (APP) gene copy number in single neurons from sporadic Alzheimer's disease brains. Bushman DM et al. (2015) Elife 4:e05116
  16. Somatic mosaicism of EPAS1 mutations in the syndrome of paraganglioma and somatostatinoma associated with polycythemia. Yang C et al (2015)Hum Genome Var. 2:15053.
  17. Induction of telomere dysfunction mediated by the telomerase substrate precursor 6-thio-2'-deoxyguanosine. Mender I et al (2015) Cancer Discov. 5(1):82-95.
  18. Role of Tet proteins in enhancer activity and telomere elongation. Lu F et al (2014)Genes Dev. 28(19): 2103–2119.
  19. Expression of the genetic suppressor element 24.2 (GSE24.2) decreases DNA damage andoxidative stress in X-linked dyskeratosis congenita cells. Manguan-Garcia C et al. (2014) PLoS One.  9(7):e101424.
  20. Telomerase Protects Werner Syndrome Lineage-Specific Stem Cells from Premature Aging. Cheung HH et al (2014) Stem Cell Reports 2(4):534–546.
  21. Rapid analysis of chromosome aberrations in mouse B lymphocytes by PNA-FISH. Misenko SM & Bunting SF (2014) journal of visualized experiments. 10.3791/51806.
  22. Effects of four chemotherapeutic agents, bleomycin, etoposide, cisplatin, and cyclophosphamide, on DNA damage and telomeres in a mouse spermatogonial cell line. Liu M et al. (2014) Biol Reprod. 90(4):72.
  23. Identification of small juvenile stem cells in aged bone marrow and their therapeutic potential for repair of the ischemic heart. Igura K,et al (2013) Am J Physiol Heart Circ Physiol. 305(9): H1354–H1362.
  24. Structure of the human telomeric Stn1-Ten1 capping complex. Bryan C et al (2013) PLoS One. 8(6):e66756.
  25. Direct DNA and PNA probe binding to telomeric regions without classical in situ hybridization. Genet MD et al (2013) Molecular Cytogenetics 6:42.
  26. Telomere and microtubule targeting in treatment-sensitive and treatment-resistant human prostate cancer cells. Zhang B et al (2012) Mol Pharmacol. 82(2):310-21.
  27. Assessing telomeric DNA content in pediatric cancers using whole-genome sequencing data. Parker M et al (2012)Genome Biol. 13(12):R113.
  28. Dynamic rearrangement of telomeres during spermatogenesis in mice. Tanemura K et al. (2005) Dev Bio281:196-207.
  29. Strand-specific FISH reveals orientation of chromosomal breaks,duplications, deletions, and numerical abnormalities in the sperm of healthy men.  Goodwin EH and Meyne J (1993) Am J Hum Genet 67:862-872.
  30. High-throughput telomere length quantification by FISH and its application to human population studies. Canela A et al (2007) Proc Natl Acad Sci USA 104(13):5300-5. (Q-FISH)
  31. Analysis of repetitive DNA in chromosomes by flowcytometry. Brind-Amour J and Lansdorp PM (2011) Nat Methods 8(6):484-6. (Q-FISH & Flow FISH)
  32. Age-related telomere length dynamics in peripheral blood mononuclear cells of healthy cynomolgus monkeys measured by Flow FISH. Lee WW et al (2002)  Immunology 105:458-65. (Q-FISH & Flow FISH)

Most FISH probes are in stock and shipped out the same day if the order is placed by 5 pm EST. 
Please email any questions to order@pnabio.com or Contact Us.

Cat No
Item
Description
Size
Price
Order
F1001

TelC-FAM

C-rich telomere probe,

FAM labeled

5 nmole
$240.00
F1002

TelC-Cy3

C-rich telomere probe,

Cy3 labeled

5 nmole
$240.00
F1003
TelC-Cy5

C-rich telomere probe, 

Cy5 labeled

5 nmole
$240.00
F1004

TelC-Alexa488

C-rich telomere probe,

Alexa Fluor 488 labeled

5 nmole
$320.00
F1005

TelG-FAM

G-rich telomere probe,

FAM labeled

5 nmole
$240.00
F1006

TelG-Cy3

G-rich telomere probe,

Cy3 labeled

5 nmole
$240.00
F1007

TelG-Cy5

G-rich telomere probe,

Cy5 labeled

5 nmole
$240.00
F1008

TelG-Alexa488

G-rich telomere probe,

Alexa Fluor 488 labeled

5 nmole
$320.00
F1009

TelC-FITC

C-rich telomere probe,

FITC labeled

5 nmole
$240.00
F1010

TelG-FITC

G-rich telomere probe,

FITC labeled

5 nmole
$240.00
F1013

TelC-Alexa647

   C-rich telomere probe,    

Alexa Fluor 647 labeled

5 nmole
$320.00
F1014

TelG-Alexa647

   G-rich telomere probe,   

Alexa Fluor 647 labeled

5 nmole
$320.00
F1020

aTelC-Alexa488

TelC probe for arthropods,

Alexa Fluor 488 labeled

5 nmole
$320.00
F2001
TelC-Biotin

C-rich telomere probe,

Biotin labeled

5 nmole
$240.00
F3001

CENPB-FAM

Centromere probe,

FAM labeled

5 nmole
$240.00
F3002

CENPB-Cy3

Centromere probe,

Cy3 labeled

5 nmole
$240.00
F3005

CENPB-Cy5

Centromere probe,

Cy5 labeled

5 nmole
$240.00
F3004

CENPB-Alexa488

Centromere probe,

Alexa Fluor 488 labeled

5 nmole
$320.00
F3008

CENPB-Biotin

Centromere probe,

Biotin labeled

5 nmole
$240.00
F3003

CENT-Cy3

Centromere probe,

Cy3 labeled

5 nmole
$240.00
F3006

CENT-FAM

Centromere probe,

FAM labeled

5 nmole
$240.00
F5001

(CAG)5-Cy3

CAG repeats,

Cy3 labeled

5 nmole
$240.00
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