Gamma/ Bis PNA

Bis PNA

Homopyrimidine PNA bind to complementary DNA forming (PNA)2/DNA triplexes of very high thermal stability via strand displacement instead of conventional triple helix formation. The most stable (PNA)2/DNA triplex is formed when the Watson-Crick base pairing PNA strand is in the anti-parallel orientation relative to the DNA strand and the Hoogsteen strand is in the parallel orientation relative to the DNA strand. Bis-PNA contains pseudoisocytosine (J) instead of cytosine (C) in the “Hoogsteen strand” can efficiently bind double-strand DNA in a pH-independent manner.

Gamma PNA

Bis PNA binding is presently limited to mostly homopurine and homopyrimidine stretches while gamma PNA can be an alternative that can be more flexible with target sequence selection.

Gamma PNA has a stereogenic center at the γ-carbon atom of the N-aminoethyl glycine unit. γ-substituted PNA can be placed in every third residue of regular PNA.

Tm of gamma PNA is higher by 5~8 ℃ per single substitution, which results in more sequence-specific binding at higher affinity. In addition, gamma PNA can invade double-stranded DNA to form a triple helix.

Moreover, gamma PNA can provide several advantages such as improved solubility, less self-aggregation, more stable PNA-DNA duplex formation, and flexibility for multi labeling and other functionalization.

  • Possible gamma functional groups
  •     - Lysine: better solubility, possible for dual-labeling, the potential for cell penetration
        - MiniPEG: best for improved solubility and specific binding, efficient for double-strand DNA invasion
        - Alanine and glutamic acid are also possible modifications.

    Custom PNA oligos provided by PNA Bio will be provided >95% purity, accompanied by COA including HPLC and mass data. Synthesis usually takes 3~4 weeks for bis PNA or gamma PNA.

    The price of custom oligo is dependent on the length, amount, and label. Please indicate the specifics in the quote request. Feel free to check out PNA Tool for Tm calculation and other guidelines for PNA design. 

    Minimum amount is 50 nmole for non-labeled PNA and 25 nmole for labeled PNA.

    Please send a quote request and order to order@pnabio.com or click here for inquire. We can also help the design of bis PNA or gamma PNA. 

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