PNA FISH Probes
PNA FISH probes hybridize to target DNA with high sensitivity and specificity mainly due to the electrical neutrality of its chemical structure. This trait enables PNA probes to be more efficient and useful in applying to FISH (fluorescent in situ hybridization) even at low concentrations. Hybridization occurs fast (within a couple of hours) and background noise is reduced significantly.
PNA Bio offers
telomere, centromere
, and CAG repeat probes as catalog items. If you need other target specific probes, we can provide a custom PNA probe synthesis service.TelC is C-rich a telomere probe for a leading strand (repeats of TAACCC) and TelG is a G-rich telomere probe for lagging strand (repeats of TTAGGG). Both TelC and TelG PNA telomere probes work with human, mouse, and rat chromosomes.
CENPB probe is a binding site for centromere protein B (CENP-B), one of the major centromere-specific proteins. The probe sequence is ATTCGTTGGAAACGGGA, and it stains minor satellite of all human and mouse centromere except the Y chromosome.
Cent probe is specific for human alpha satellite centromere and does not react to the mouse.
The sequence of the probe is AAACTAGACAGAAGCAT.
For FISH protocol using PNA FISH probes, please download the following file.
The sequence of the probe is AAACTAGACAGAAGCAT.
For FISH protocol using PNA FISH probes, please download the following file.
F1001 (TelC-FAM)
F1002 (TelC-Cy3)
F1002 & F3001 (CENPB-FAM)
F1006 (TelG-Cy3)
F3006 (Cent-FAM)
F5001 (CAG5-Cy3)| Catalog Number | Item | Label | Information |
| F1001 | TelC-FAM | FAM | TelC telomere probe
(CCCTAA repeats)C-rich / leading strand Reacts to human, mouse, rat, etc |
| F1002 | TelC-Cy3 | Cy3 | |
| F1003 | TelC-Cy5 | Cy5 | |
| F1004 | TelC-Alexa488 | Alexa Fluor 488 | |
| F1009 | TelC-FITC | FITC | |
| F1013 | TelC-Alexa647 | Alexa Fluor 647 | |
| F2001 | TelC-Biotin | Biotin | |
| F1005 | TelG-FAM | FAM | TelG telomere probe
(TTAGGG repeats)G-rich / lagging strand Reacts to human, mouse, rat, etc |
| F1006 | TelG-Cy3 | Cy3 | |
| F1007 | TelG-Cy5 | Cy5 | |
| F1008 | TelG-Alexa488 | Alexa Fluor 488 | |
| F1010 | TelG-FITC | FITC | |
| F1014 | TelG-Alexa 647 | Alexa Fluor 647 | |
| F2002 | TelG-Biotin | Biotin | |
| F3001 | CENPB-FAM | FAM | Pan-centromere probe
(ATTCGTTGGAAACGGGA)Reacts to human & mouse |
| F3010 | CENPB-FITC | FITC | |
| F3002 | CENPB-Cy3 | Cy3 | |
| F3004 | CENPB-Alexa488 | Alexa Fluor 488 | |
| F3005 | CENPB-Cy5 | Cy5 | |
| F3008 | CENPB-Biotin | Biotin | |
| F3009 | CENPB-RC-Cy3 | Cy3 | |
| F3003 | CENT-Cy3 | Cy3 | Human only pan-centromere probe
(AAACTAGACAGAAGCATT) |
| F3006 | CENT-FAM | FAM | |
| F3007 | CENT-RC-A488 | Alexa488 | |
| F5001 | (CAG)5-Cy3 | Cy3 | Stains CAG repeats (DM-1) |
| F5002 | (CAG)5-Cy5 | Cy5 | |
* FAM and Alexa488 is more stable alternative for FITC.
* Cy3 is more stable alternative for Texas Red, TRITC, TMR and other red dyes.
- Integrity of the human centromere DNA repeats is protected by CENP-A, CENP-C, & CENP-T . Giunta S & Funabiki H. (2017) Proc Natl Acad Sci USA 114(8):1928-1933.
- Comprehensive characterization of neutrophil genome topology. Zhu Y et al. (2017) Genes & Development. 31(2):141–153.
- Long telomeres protect against age-dependent cardiac disease caused by NOTCH1 haploinsufficiency. Theodoris CV et al. (2017) J Clinical Investigation 127(5):1683-1688.
- Quantification of telomere features in tumor tissue sections by an automated 3D imaging-based workflow. Gunkel M et al (2017) Methods 114:60-73.
- Telomere Replication Stress Induced by POT1 Inactivation Accelerates Tumorigenesis. Pinzaru AM et al. (2016) Cell Rep 15(10):2170-2184.
- Regulation of the Human Telomerase Gene TERT by Telomere Position Effect—Over Long Distances (TPE-OLD): Implications for Aging and Cancer. Kim W et al (2016) pLoS Biology 2000016.
- Dysfunctional telomeres induce p53‐dependent and independent apoptosis to compromise cellular proliferation and inhibit tumor formation. Wang Y et al (2016) Aging Cell 15(4): 646–660.
- ATRX loss promotes tumor growth and impairs nonhomologous end joining DNA repair in glioma. Koschmann C et al (2016) Sci Transl Med. 8(328):328.
- Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis. Elkouby YM et al. (2016) PLoS Biol. 14(1):e1002335.
- Cell Death During Crisis Is Mediated by Mitotic Telomere Deprotection. Hayashi MT et al (2015)Nature 522(7557):492–496.
- SMARCAL1 maintains telomere integrity during DNA replication. Poole LA et al. (2015) Proc Natl Acad Sci U S A. 112(48):14864-14869.
- Visualization and quantitative analysis of extrachromosomal telomere-repeat DNA in individual human cells by Halo-FISH. Komosa M et al. (2015)Nucleic Acids Res. 43(4):2152–2163.
- Flap Endonuclease 1 Limits Telomere Fragility on the Leading Strand.Teasley DC et al (2015) J Biol Chem. 290(24):15133-45.
- Replication Stress and Telomere Dysfunction Are Present in Cultured Human Embryonic Stem Cells. Janson C et al (2015) Cytogenet Genome Res. 146(4):251-60.
- Genomic mosaicism with increased amyloid precursor protein (APP) gene copy number in single neurons from sporadic Alzheimer's disease brains. Bushman DM et al. (2015) Elife 4:e05116.
- Somatic mosaicism of EPAS1 mutations in the syndrome of paraganglioma and somatostatinoma associated with polycythemia. Yang C et al (2015) Hum Genome Var. 2:15053.
- Induction of telomere dysfunction mediated by the telomerase substrate precursor 6-thio-2'-deoxyguanosine. Mender I et al (2015) Cancer Discov. 5(1):82-95.
- Role of Tet proteins in enhancer activity and telomere elongation. Lu F et al (2014)Genes Dev. 28(19): 2103–2119.
- Expression of the genetic suppressor element 24.2 (GSE24.2) decreases DNA damage andoxidative stress in X-linked dyskeratosis congenita cells. Manguan-Garcia C et al. (2014) PLoS One. 9(7):e101424.
- Telomerase Protects Werner Syndrome Lineage-Specific Stem Cells from Premature Aging. Cheung HH et al (2014) Stem Cell Reports 2(4):534–546.
- Rapid analysis of chromosome aberrations in mouse B lymphocytes by PNA-FISH. Misenko SM & Bunting SF (2014) Journal of visualized experiments 10.3791/51806.
- Identification of small juvenile stem cells in aged bone marrow and their therapeutic potential for repair of the ischemic heart. Igura K et al (2013) Am J Physiol Heart Circ Physiol. 305(9): H1354–H1362.
- Structure of the human telomeric Stn1-Ten1 capping complex. Bryan C et al (2013) PLoS One 8(6):e66756.
- Direct DNA and PNA probe binding to telomeric regions without classical in situ hybridization. Genet MD et al (2013) Molecular Cytogenetics 6:42.
- Telomere and microtubule targeting in treatment-sensitive and treatment-resistant human prostate cancer cells. Zhang B et al (2012) Mol Pharmacol. 82(2):310-321.
- Assessing telomeric DNA content in pediatric cancers using whole-genome sequencing data. Parker M et al (2012) Genome Biol. 13(12):R113.
- Dynamic rearrangement of telomeres during spermatogenesis in mice. Tanemura K et al. (2005) Dev Bio 281:196-207.
- High-throughput telomere length quantification by FISH and its application to human population studies. Canela A et al (2007) Proc Natl Acad Sci USA 104(13):5300-5. (Q-FISH)
- Analysis of repetitive DNA in chromosomes by flow cytometry. Brind-Amour J and Lansdorp PM (2011) Nat Methods 8(6):484-6.(Q-FISH & Flow FISH)
- Age-related telomere length dynamics in peripheral blood mononuclear cells of healthy cynomolgus monkeys measured by Flow FISH. Lee WW et al (2002) Immunology 105:458-65. (Q-FISH & Flow FISH)
- Single base discrimination of CENP-B repeats on mouse and human Chromosomes with PNA-FISH. Chen C et al (1999) Mammalian Genome 10(1):13–18.
Most FISH probes are in stock and shipped out the same day if the order is placed by 5 pm EST.
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